Jeffrey S. Patrick | Authors

The application of high-resolution TOF-MS to petroleomics is presented, and the basic theory of each type of ultrahigh-resolution mass spectral platform is briefly explained.

A proof-of-concept application for detecting abused steroids in urine samples collected from an antidoping surveillance program

High performance time-of-flight mass spectrometry (TOF-MS) is applied to the analysis and characterization of complex biological samples. High mass accuracy, high resolution, and accurate relative isotope abundance are all applied to the determination of analytes covering a range of concentrations in complex matrices including plasma and urine. Qualitative and quantitative evaluations are provided and include demonstrations of the impact of high-performance MS on sensitivity and selectivity. The ability to leverage high-performance MS in conjunction with a broad dynamic range in rapid ultrahigh-pressure liquid chromatography (UHPLC) analyses to identify unknowns and to propose putative metabolic biomarkers is demonstrated. The impact of speed of analysis and selectivity to the depth of coverage and accuracy of the analyses are discussed.

The impact of speed of analysis and selectivity to the depth of coverage and accuracy of the analyses are discussed.