John W. Henderson Jr. | Authors


Fast Separation of Seven Biocides Using an Agilent ZORBAX RRHD Eclipse Plus C18 1.8 µm Column

The flexibility to use short sub-2 micron columns on UHPLC instruments at high flow-rates permits fast analysis with high resolution of complex samples. An Agilent ZORBAX Rapid Resolution High Definition (RRHD) Eclipse Plus C18 1.8 µm column separated seven different biocides in less than 1 minute with high resolution. A flow-rate of 1.7 mL/min was used on 2.1 × 50 mm column to achieve the rapid separation. The Agilent 1290 Infinity LC system was used as the column pressure just exceeded 1000 bar at this high flow-rate. A sample of hand sanitizer was found to contain 2-phenoxyethanol and methylparaben using the method.

Stevia Sweetener Analyses Using an Agilent ZORBAX Eclipse Plus Phenyl-Hexyl Column

An Agilent ZORBAX Rapid Resolution HT Eclipse Plus Phenyl-Hexyl column resolved many compounds from Stevia rebaudiana Bertoni plant extracts using an isocratic reversed phase method with UV and MS detection instead of a typical approach employing an amino column with a refractive index or other special detector. The Eclipse Plus Phenyl-Hexyl method's advantages include high peak capacity, isocratic mobile phase, low acetonitrile consumption, and MS compatibility.

Comparing Selectivity of Phenylhexyl and Other Types of Phenyl Bonded Phases

A new phenylhexyl bonded phase provides an alternative selectivity for optimizing HPLC method. This also applies when different types of phenyl bonded phases are compared.