Slalom chromatography speeds mRNA QC—separating plasmid forms and spotting dsRNA impurities in minutes with higher sensitivity than gels or ELISA.
Matthew A. Lauber
Advertisement
Matthew Lauber is a Senior Director within Waters (Corporation, Milford, USA) where he oversees the development of new columns, reagents and enzymes for the analysis of biomolecules. He has been a leading inventor in this field of work for over a decade having published 80 research articles on LC based techniques.
Articles by Matthew A. Lauber
Hydrophilic interaction liquid chromatography (HILIC) has emerged as a promising alternative to traditional ion-pair reversed phase liquid chromatography (IP-RPLC) methods for separating oligonucleotides (ON). This work investigates the application of HILIC to the separation of ON sequence and length variants, duplexes, and single-stranded components.
New gene therapy modalities, such as CRISPR guide RNA (single guide ribonucleic acid [sgRNA]) and messenger RNA (mRNA), continue to make progress in both primate and first-in-human trials. As this progress builds, the industry remains accountable for characterizing these molecules to meet the requirements of regulatory authorities.
Pressure-enhanced liquid chromatography (PE-LC) offers a new approach for improving selectivity for large molecule separations. Examples shown here include short oligonucleotides in ion-pairing reversed-phase (IP-RP) liquid chromatography and larger nucleic acids in ion-exchange (IEX) chromatography.
Advertisement
Latest Updated Articles
Advertisement
Advertisement
Trending on LCGC International
1
Advanced Gas Chromatography Methods for Detecting PFAS Contamination in Soil
2
More Tools for Sample Injection When Faced with Mobile Phase/Sample Solvent Mismatch
3
LC–MS/MS Detects MCI/MI in Danish Soaps and Shampoos
4
The Analytical Challenge: Detection, Quantification, and Method Development
5