OR WAIT 15 SECS
© 2020 MJH Life Sciences and Chromatography Online. All rights reserved.
© 2020 MJH Life Sciences™ and Chromatography Online. All rights reserved.
The application of a novel approach that enables a selective proteolysis of the Fab region of monoclonal antibodies (mAbs) is described. The technique is called nano-surface and molecular-orientation limited (nSMOL) proteolysis. By restricting the proteolysis to the complementarity-determining region (CDR) of the mAb, there is considerable reduction in sample complexity and the time required for method set-up and optimization.