Paul Rammage | Authors


Asymmetric Flow Field-Flow Fractionation (AF4) with Multi-Angle Light Scattering (MALS) for High-Throughput Protein Refolding

One of the biggest problems facing researchers involved in pharmacogenomics is analysing the recombinant proteins of interest to monitor if they are in a folded state. This article describes a rapid and economical method using asymmetric flow field-flow fractionation combined with multi-angle light scattering (AF4–MALS) to characterize refolded proteins, which overcomes some of the disadvantages associated with other techniques.