Metal-free columns should be considered when troubleshooting ion suppression. Chelating compounds that can form metal salt with stainless steel column housings are examined.
One of the fundamental tenets of chromatography is the interaction between an analyte and stationary phase.
Polar neurotransmitters are difficult to retain using ODS columns. In this work we show two different methods to retain these polar analytes on the multi-mode Scherzo SS-C18 column. The versatility in retention characteristics with simple changes in mobile phase is highlighted.
The challenging retention of polar organic acids in reversed phase conditions is accomplished using a unique multi-mode stationary phase.
The Intrada Amino Acid column has been shown to be a viable solution for the analysis of amino acids by LC–MS without derivatization.