Using Earth as a figurative analogy, we explore the merits of flexibility when considering which liquid chromatography (LC) modality to use for any particular bioanalysis.
A novel approach using hydrophilic-phase extraction (HPE) for the analysis of oligonucleotides in bioanalysis applications is described. The HPE method was proven to be a critical part of a fully quantitative HILIC–high-resolution mass spectrometry (HRMS) method for the RNA oligonucleotide and has potential for general application.