This article will discuss an ion-pair reversed-phase liquid chromatography mass spectrometry (IP-RPLC–MS) workflow for the characterization of three messenger RNA (mRNA) key critical quality attributes: 5’ Cap, ORF, and poly(A) tail.
Roxana Eggleston-Rangel
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Roxana Eggleston-Rangel is an application scientist at Phenomenex. She received her bachelors and masters degree in chemistry and biochemistry from California State University (Los Angeles, USA). Roxana is an LC–MS/MS enthusiast currently working on developing LC–MS/MS methods, with a focus on nano- and micro-flow separations.
Articles by Roxana Eggleston-Rangel
The evolution of LC–MS for characterizing mRNA therapeutics is discussed.
Reducing the column inner diameter results in a reduction in chromatographic dilution and increased sensitivity, allowing for more efficient tandem mass spectrometry sampling (MS/MS) and a higher number of molecule identifications.
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