LCGC Europe-03-01-2020

Click the title above to open the LCGC Europe March 2020 regular issue, Volume 33, Number 3, in an interactive PDF format.

Mobile phase flow is critically important, and must be carefully controlled. In this instalment of “GC Connections”, we discuss carrier gas flow and its importance in successful gas chromatographic (GC) analysis. We will begin with a short review of fundamental theories showing why flow is important, and move into a discussion of how flow rate is measured and controlled on modern instruments. Finally, we will discuss the effect of flow-related parameters, including carrier gas choice, the difference between constant pressure and constant flow modes, and some new thinking about flow rate optimization. We conclude with some takeaways that should assist gas chromatographers with effective carrier gas management.

The effect of carrier liquid composition on the molecular properties of uncross-linked and enzymatically cross-linked caseinate solutions was studied by asymmetrical flow field-flow fractionation (AF4).

How do bioinert and biocompatible liquid chromatography (LC) systems and columns improve separations of biomolecules? How do I know when these systems are required for my separation?

This article will discuss the benefits of 2D-LC and multiple application areas in (bio)pharmaceutical analysis, and will highlight the challenges and future outlook.