The State of The Art in Oligonucleotide Analysis: An LCGC International Peer Exchange

The panel introduces themselves and provides some background on their experience with oligonucleotide analysis.

The main focus of this Peer Exchange is on the state of the art for antisense oligonucleotides (ASOs) in particular. The panel shares their perspectives on the most pressing analytical challenges the industry currently faces regarding these oligonucleotides.

There is a broad range of chemistries available for the analysis of oligonucleotides, and the panel narrows in on stationary phases and column materials relevant to ASOs.

This episode covers strategies in place for the analysis of impure mixtures with a larger range of retention factors while still maintaining separation power.

The panel dives into the regulatory stance on oligonucleotide enantiomers. The role of batch-to-batch reproducibility, new drug application (NDA) filing, and current guidelines are covered.

In addition to the current state of the art in oligonucleotide analysis, the panel turns to discussing the future of the industry and separation power.

Is there a competency crisis in the industry? The panel shares their thoughts on how robustness and reproducibility connect.

Based on their experiences, the panel discusses on-column structural formations.

Fluorinated compounds have become prevalent in ion pair chromatography (IPC) but they do pose some environmental concerns.

This episode focuses on alternative modes of liquid chromatography (LC) for oligonucleotides as current go-to chromatographic modes may face significant changes in the future.