The panel considers how AI and automation could reshape peptide method development, and why scientific judgment will remain essential.
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Piotr Alvarez explains how HILIC complements reversed-phase LC for GLP-1 peptides, and why the field is moving toward MS-friendly TFA alternatives.
Panelists identify the parameters that make or break peptide method robustness and tech transfer, including temperature, flow rate, and dwell volume.
The panel discusses quantitative peptide analysis, including drug substance vs. drug product complexity, recovery, and methionine oxidation risk.
The panel cover protecting peptide recovery, from mobile-phase pH and diluent buffers to inert column hardware.
Piotr Alvarez explains how his lab uses 2D-LC to enable MS compatibility with legacy methods and to confirm peak purity through orthogonal separation.
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