20 Steps to a Reliable Mobile Phase



Webinar Date/Time: Thursday, February 16th, 2023 at 8am PST | 11am EST | 4pm GMT | 5 pm CET

Selection of mobile phase and correct preparation is essential for a robust HPLC method. This webcast will provide a failsafe 20-step guide to the design and preparation of the optimum mobile phase; this includes controlling pH, eluent mixing, filtration and degassing, mobile phase shelf life, and some of the most common mistakes to avoid.

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Event Overview:

HPLC mobile phase is at the heart of an HPLC method. It is used to control the retention of analytes and, if chosen or prepared incorrectly, can cause a myriad of problems such as extra peaks, unexplained retention time shifts, and system contamination. These top 20 tips for successfully designing and preparing a mobile phase will help individuals avoid many of the common pitfalls that occur while using this method.

Topics Covered:

  • Correctly controlling pH using buffers
  • Eluent mixing
  • Filtration and degassing
  • Reagent quality
  • Mobile phase shelf life
  • Impact of mobile phase in gradient HPLC
  • System flushing
  • Common mistakes to avoid


Colin Towers
Senior Technical Support Chemist

After completing a degree in pharmacy, Colin Towers pursued a career in analytical chemistry. Following three years performing routine testing and three years instrument maintenance and calibration, he moved into a role as a method development and validation specialist in contract research. Gaining 11 years’ experience in developing methods for HPLC, LC-MS, GC, GC-MS and SPE in a high throughput commercial environment, this was supplemented by three years lecturing in forensic analytical toxicology and a further three years working as a community pharmacist. Towers has now been with Crawford Scientific for eight years as a technical specialist, consultant, and trainer.

Dr. Dawn Watson
CHROMacademy Product Manager

Dr. Watson completed her Ph.D. in synthetic inorganic chemistry at the University of Strathclyde, Glasgow, followed by postdoctoral research on small molecule reaction kinetics at Princeton University. Following several years working for a major instrument manufacturer, she became a technical expert for CHROMacademy in 2013. She has expertise in various analytical techniques, including HPLC, GC, GC–MS, LC–MS, NMR, and molecular spectroscopy, as well as numerous wet chemistry and sample preparation techniques.

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