Alasdair Matheson

At HPLC 2025 in Bruges, Belgium LCGC International interviewed a member of our editorial advisory board (EAB), Paul Ferguson, a leading voice in sustainability in the pharmaceutical sector, to explore the intersection of green chemistry and emerging therapeutics.

LCGC International interviewed Professor Torgny Fornstedt, Professor in Analytical Chemistry & Leader of the Fundamental Separation Science Group at Karlstad, Sweden at HPLC 2025 in Bruges, Belgium.

LCGC International interviewed Torgny Fornstedt, Professor in Analytical Chemistry & Leader of the Fundamental Separation Science Group at Karlstad University, Sweden at HPLC 2025 in Bruges, Belgium on modern separation methods to analyse oligonucleotides.

Bo Zhang, associate professor at Xiamen University, China, spoke to LCGC International at HPLC 2025 in Bruges, Belgium, about his recent research on a novel elastic connection technology—macroporous polyacrylamide hydrogel septum (MAPS)—which was developed as a complementary solution for capillary column interfacing in liquid chromatography.

LCGC International interviewed Bob Pirok from the University of Amsterdam, Netherlands to discuss strategies for enhancing method robustness in 2D LC, practical approaches for tracking peaks across complex data sets, and the role of machine learning and AI in chromatography.

Schug discusses the role of surrogate modelling in chromatographic method development and process optimization.

Schug discusses the application and benefits of vacuum ultraviolet (VUV) detection in gas chromatography (GC) and how advanced modern machine learning approaches can be implemented.

In this interview, Caroline West, professor at the University of Orléans and an active member of LCGC International’s editorial advisory board, discusses current perspectives on the fundamentals of SFC.

In this interview, Gesa Schad from Shimadzu Europe, Germany, discusses how SFC is being used in practice, why she launched SFC For Dummies at HPLC 2025, and how common misconceptions surrounding the robustness of SFC have been addressed some time ago.

In this video interview with Peter Schoenmakers and Bob Pirok, they discuss their new textbook, the future of separation science, and how their book can empower the old, the new, and the next generation of analysts and researchers.

Martin Vollmer, biopharma program manager at Agilent Technologies, spoke to LCGC International about the evolving importance of glucagon-like peptide-1 (GLP-1) therapeutics and the challenges they present to separation scientists.

A recap of the afternoon session focused on pharmaceutical separations, from Day 2 of HPLC 2025.

A recap of the morning session focused on method development, from Day 2 of HPLC 2025.

Highlights from the afternoon sessions of HPLC 2025: Oligonucleotide analysis

Highlights from the opening session and the first day of HPLC 2025

In an age of information overload LCGC International’s mission is to deliver practical, unbiased educational content to help separation scientists perform more effectively and efficiently in the workplace.

The 54th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2025) will be held from Sunday through Thursday, June 15–19 2025, in Bruges, Belgium. LCGC International spoke to the Scientific Committee to find out what’s in store.

Fabrice Gritti, consultant scientist at Waters Corporation, spoke to LCGC International about the history of slalom chromatography (SC) and why he decided that the technique was worth re-investigating. The potential benefits of SC, according to Gritti, include identifying RNA impurities when manufacturing mRNA therapeutics, and assisting in the development of new gene and cell therapies, and other biopharmaceutical applications.

LCGC International spoke to Fabrice Gritti, consultant scientist at Waters Corporation, about the history of slalom chromatography (SC) and why he decided that the technique was worth re-investigating.

LCGC International spoke to Craig Wheelock and Matthew Smith from the Wheelock Laboratory at the Karolinska Institute, Sweden, about a novel desorption electrospray ionization–multiple-reaction-monitoring mass spectrometry (DESI-MRM-MS) workflow his team has developed to spatially map oxylipins in pulmonary tissue.

The critical role of separation science in per- and polyfluoroalkyl substances (PFAS) research highlights just how essential chromatography is in solving complex challenges that benefit society.

In a commentary published in Environmental Health Perspectives, researchers voiced concerns about the limitations in PFAS research due to restricted access to high-purity reference standards.

In a commentary published in Environmental Health Perspectives, researchers voiced concerns about the limitations in PFAS research due to restricted access to high-purity reference standards.

Catharina Capitain and Melanie Schüßler from the Faculty of Geosciences at the University of Tübingen, Tübingen, Germany describe a novel approach using matrix-matched semiquantification to investigate per- and polyfluoroalkyl substances (PFAS) in contaminated soil.

Oliver Jones and Navneet Singh from RMIT University, Melbourne, Australia discuss a recent study they conducted to investigate the relationship between tidal cycles and PFAS concentrations in estuarine systems, and offer practical advice on the sample preparation and LC–MS/MS techniques they used to achieve the best results.

LCGC International spoke to Lauren Lovejoy, principal scientist in the Drug Substance and Product Analysis group at GSK (Philadelphia, Pennsylvania), about the advantages of using tandem columns in sequential elution liquid chromatography (SE-LC) to investigate weak acids, neutral compounds, and permanent anions in pharmaceutical applications.

Joel Fabregat-Palau, a postdoctoral researcher at the University of Tübingen (Germany) discusses his latest research investigating per- and polyfluoroalkyl substances (PFAS) in agricultural soils using a targeted approach with high performance liquid chromatography tandem mass spectrometry (HPLC–MS/MS) and a non-targeted high performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC–QTOF-MS) for semi-quantification.

David Megson from Manchester Metropolitan University in Manchester, UK, spoke to LCGC International about the latest developments in non-targeted analysis (NTA) of per- and polyfluoroalkyl substances (PFAS) in environmental matrices based on a recent systematic review paper he has collaboratively published (1).

Emanuela Gionfriddo and Madison Williams from University at Buffalo, the State University of New York, NY, USA discuss the important role that solid-phase microextraction (SPME) techniques with gas chromatography mass spectrometry (GC–MS) can play in the analysis of per- and polyfluoroalkyl substances (PFAS).