Analysis of Small Molecules with amaZon: Enhanced Sensitivity, Speed, and Dynamic Range

The Application Notebook

The Application Notebook, The Application Notebook-06-01-2010, Volume 0, Issue 0

The amaZon series is the next step in Bruker Daltonics' family of ion trap mass spectrometers. Incorporating a number of new innovations to Bruker's highly successful ion trap technology platforms, the amaZon series is a true leap forward in performance and reliability.

The amaZon series is the next step in Bruker Daltonics' family of ion trap mass spectrometers. Incorporating a number of new innovations to Bruker's highly successful ion trap technology platforms, the amaZon series is a true leap forward in performance and reliability.

Figure 1

Building upon Bruker's vast foundation of expertise with ion traps, the amaZon series of instruments features a number of key performance enhancements including:

  • Greater than 10× sensitivity improvement over previous generation systems

  • Outstanding mass resolution of up to 20,000

  • Extraordinarily broad mass range from 50 to 3,000 m/z

  • Rapid scan speeds of up to 52,000 µ/s allowing full compatibility with fast separation techniques

  • The very latest in ETD/PTR technology for analysis of post translational modifications of peptides or proteins

  • High speed ion polarity switching for handling difficult or complex samples

  • Comprehensive MSn spectral libraries for easy molecular formula determinations

The amaZon series is available in two versions: amaZon ETD for proteomics amaZonX for all small molecule applications

Designed for key applications in proteomics, metabolomics, screening, and compound identification, the amaZon series is the top performing trap available today.

Figure 2

The performance of the amaZonX ion trap system has been tested in terms of sensitivity, speed, and dynamic range for small molecule analysis. It has been shown that the amaZon is easily capable of detecting low femtograms of a small molecule on column. The amaZon also displays at least four orders of magnitude dynamic range when quantitating small molecules in the ppb to ppm range. The instrument also possesses the capability to do "Zero Delay" polarity switching, even at UPLC separation speeds. All of these attributes demonstrate the high performance capabilities of the amaZon and demonstrate its suitability for deployment in a number of small molecule analysis areas.

Figure 3

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