|Articles|December 3, 2019

Analytical Quality-by-Design Based Method Development for the Analysis of Formoterol, Budesonide, and Related Compounds Using UHPLC-MS

⦁ Develop a high performance and robust method for the analysis of formoterol, budesonide, and related compounds. ⦁ Show the straightforward method development capabilities of the ACQUITY™ Arc™ System in combination with Empower™ 3 Chromatographic Data System and S-Matrix Fusion QbD® Software.


Related to this article

Chromatography Detects Toxins in Tea Samples
High-performance liquid chromatography-tandem mass spectroscopy (HPLC–MS/MS) chromatography reveals pyrrolizidine alkaloids (PA)/pyrrolizidine alkaloid N-oxides (PANO) toxin risks in 63 tea samples.
Figure 2. Split injection used to measure triplicate test of MTBE, hexane, o-xylene, and 1-methylnaphthalene comparing different solvents (methanol, methylene chloride) and column insertion distances starting with 0.5 mm, 5.0 mm, 10.0 mm, 15 mm, and 20.0 mm. Methanol had the best performance at the 5.0 mm insertion distance and methylene chloride looked slightly better at the 0.5 mm. We would still recommend not going below the 5.0 mm manufacturer recommended insertion distance.
Revisiting insertion distance for methanol and methylene chloride in GC analysis and confirming that insertion depth strongly affects response and reproducibility.