Base Hydrolysis and Accelerated Solvent Extraction (ASE): Determination of Total Fat from Dairy Products

Brett Murphy, Jennifer Peterson, Bruce Richter, David Knowles, and Richard Carlson, Dionex Corporation

This application note demonstrates the extraction of sour cream, cream cheese, coffee creamer, heavy whipping cream, and low fat milk using an ASE^® 350 Accelerated Solvent Extractor technique developed by Dionex and compares results to the traditional Mojonnier technique. The resulting extracts were derivatized and analyzed by GC–MS according to AOAC Official Method 996.06.

Previously, it has been shown that ASE instruments can successfully extract fat from a number of different food matrices after an acidic hydrolysis treatment step (1). This application note demonstrates that ASE can also be used to extract dairy products following base hydrolysis. Current methods for determining fat in dairy products are adequate, but have several drawbacks: they are very labor intensive and time consuming, and they consume large amounts of expensive solvents. Dairy products present a set of complex matrices. Pretreatment steps are required to denature the casein, allowing greater exposure of the fat to the extraction solvent. For example, a cheese sample must be treated in a multiple-step process even before the solvent extraction can be carried out. The entire manual extraction process usually requires 2–3 h and more than 100 mL of solvent per sample (AOAC Official Method 933.05). Although standard fat extraction methods — such as the Roese-Gottlieb, Gerber, Babcock, and Mojonnier methods — give satisfactory results, they are time consuming and labor intensive.

Experimental Conditions

Sample Preparation:

Dairy samples were prepared by first performing a base hydrolysis according to AOAC Official Method 996.06 section G. The sample mixture was then mixed with 22 g of ASE Prep CR H+ and 12 g of ground ASE Prep DE. This sample mixture was then added to a 100 mL Dionium™ extraction cell containing an additional 5 g ASE Prep CR H+. The extraction cell was placed onto the ASE instrument and extracted using the following method:

ASE Conditions:

Cell Size: 100 mL

Cell Type: Dionium

Pressure: 1500 psi

Temperature: 100 °C

Solvent: Hexane

Static Time: 5 min

Static Cycles: 3

Flush: 60%

Purge: 150 s

Table 1

Results

The extracts were evaporated to dryness for gravimetric fat determination and the remaining fat was esterified for FAME analysis using AOAC Official Method 996.06 section G and were analyzed using GC–MS.

Conclusions

Combined with base hydrolysis, ASE yields equivalent results for determination of lipids from dairy products, when compared to more time-consuming extraction techniques such as the Mojonnier method.

Reference

(1) Dionex Corporation. Extraction of Total Fat from Food Samples After Acid Hydrolysis Using Accelerated Solvent Extraction (ASE) with GC-MS Analysis. Application Note 361 (LPN 2008, March 2008) Sunnyvale, CA.

Dionex Corporation

1228 Titan Way, P.O. Box 3603, Sunnyvale, CA 94088

tel. (408)737-0700, fax (408) 730-9403

Website: www.dionex.com