The Application Notebook
Red wine is a challenging matrix that contains organic acids, sugars, phenols, and pigments such as anthocyanins. This application note describes a simple, fast, novel, and effective method to clean up red wine co-extractives.
Red wine is a challenging matrix that contains organic acids, sugars, phenols, and pigments such as anthocyanins. This application note describes a simple, fast, novel, and effective method to clean up red wine co-extractives.
a) Add 10 mL red wine to 50 mL tube (RFV0050CT)
b) Add 10 mL acetonitrile, vortex 30 s
c) Add salts from Mylar pouch (ECQUUS2-MP)
d) Shake vigorously for 1 min
e) Centrifuge at 5000 rpm for 5 min
f) Supernatant is ready for cleanup
Table I: Extraction and clean-up materials
a) Load 1 mL supernatant with disposable syringe
b) Push the supernatant slowly through the cartridge (ECPURMPSMC)
c) Collect 0.5 mL extract into 2 mL autosampler vial
d) Add triphenyl phosphate as internal standard
Figure 1: Left: quick QuEChERS cartridges before and after cleanup of 1 mL red wine extract; right: red wine extract before (left) and after (right) Quick QuEChERS cleanup.
LC: Thermo Accela 1250 pump
B: 0.1% formic acid in methanol
8 min: 5% A; 14 min: 5% A; 14.2 min: 95% A;
16 min: 95% A
Table II: SRM transitions
MS/MS: Thermo TSQ Vantage
Recoveries ranging from 81.6% to 112.2% with RSD less than 10.8% were achieved for red wine samples fortified with 10, 50, and 100 ng/mL of pesticides.
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