The Effectiveness of Polar Stationary Phase Modification on Peak Shape for Basic Compounds Under General Reversed Phase Conditions – A Comparison of Four Alkyl C18 Phases

HPLC is the method of choice for enhancing the purity of synthesized peptides. Here, a systematic and efficient workflow for scaling up peptide purification is shown.

In this study, the unique capabilities of a LC-UV/MS workflow to accelerate analysis are demonstrated in the identification of impurities related to GLP-1 analogs that underwent chemical and thermal stress conditions.

In this study, we present a QC-friendly LC-UV/MS workflow designed to alleviate the workload on analytical laboratories. Access to orthogonal mass data allows supporting labs to make quicker and more informed decisions during method development, reducing errors, and increasing overall productivity.

This method provides precise separation with sharp and symmetric peaks for the coordinating compounds of the kynurenine pathway.