GC Troubleshooting — A Troubleshooter's Tool Kit

This LCGC Blog installment explains how NIST categorizes and certifies reference materials, and why they underpin reliable results in science and medicine.

Comparing manual and automated N-glycan profiling of a trastuzumab biosimilar, researchers found that both workflows detected the same glycan types, but automation gave more reproducible results (mean CV of about 5.7% vs. 7.95%), making it better suited to high-throughput analysis in biopharmaceutical development.

How can analysts resolve impurities that differ by a single mass unit? Experts discuss deamidation, racemization, HRMS, and 2D-LC for GLP-1 peptides.

"LC Troubleshooting" explores why phosphorothioate oligonucleotides (ONs) diastereomers challenge liquid chromatography.

This new high-performance liquid chromatography (HPLC) method reliably measures cholesterol adsorption in artichoke fiber.

The panel walks through confirming peptide identity–from HRMS and LC–MS/MS sequencing to amino acid analysis and D/L-isomer verification.

Malte Hübschen of the University of Duisburg-Essen (Germany) discusses an automated gas chromatography-tandem mass spectrometry (GC-MS/MS) workflow that improves phthalate detection in tricky oils with LCGC International.

Kinjal Bhatt says belonging comes from team culture, not headcount, while Ina Varfaj's goal as her lab's only woman is making sure she isn't the last.