|Articles|September 16, 2019

High Recovery of Peptides Using Strata-X PRO Solid-Phase Extraction

For this peptide application, two different types of polymeric SPE (WCX and SAX) were tested in parallel with Strata-X PRO SPE and compared in regards to recovery and % variation.


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Figure 2. Split injection used to measure triplicate test of MTBE, hexane, o-xylene, and 1-methylnaphthalene comparing different solvents (methanol, methylene chloride) and column insertion distances starting with 0.5 mm, 5.0 mm, 10.0 mm, 15 mm, and 20.0 mm. Methanol had the best performance at the 5.0 mm insertion distance and methylene chloride looked slightly better at the 0.5 mm. We would still recommend not going below the 5.0 mm manufacturer recommended insertion distance.
Revisiting insertion distance for methanol and methylene chloride in GC analysis and confirming that insertion depth strongly affects response and reproducibility.