This application note illustrates a high-sensitivity method for the analysis of fluticasone propionate in rat plasma using ACQUITY UPLC and Xevo TQ MS. High sensitivity was achieved using just 375 uL of plasma in a rapid-throughput assay. The rapid data capture of the Xevo TQ MS allowed for the narrow MRM peaks to be accurately defined and quantified.
Transferring Methods to Compact and Portable HPLC
February 14th 2024The current trend in laboratory equipment design is the miniaturization of laboratory instruments. Smaller-scale HPLC instruments offer benefits that cannot be matched by analytical-scale equipment, especially in the areas of portability, reduced fluid volumes, and reduced operating costs. Yet, the miniaturization of laboratory equipment has brought with it a unique set of challenges, including transferring methods to compact LC. Capillary LC expands the use of LC to applications not currently done using conventional LC in a wide array of application areas, including pharmaceutical, food and beverage, petrochemical, environmental, and oil and gas. Greg Ward, Axcend’s CEO wrote, “Customers want an HPLC system with a small footprint, low flow rates and green chemistry.” Join his podcast where he shares method transfer in these application areas.