HPLC 2023 New Anion-Exchange Columns for the Analysis of N-Glycans Using HPAEC-PAD/MS

Separation of all 6 glycan monosaccharides of interest and 2-Deoxy-D-Glucose (glycosylation inhibitor) was achieved within 7 minutes using the new SweetSep™ AEX20 column under isocratic elution conditions. Furthermore, profiling of released N-glycans from glycoproteins without the need for sample derivatization is shown using HPAEC-MS.