HPLC for Practicing Scientists: A Brief History of the Technique

LCGC International’s “Perspectives in HPLC” column editor Michael Dong, recently completed a 26-episode video series, “HPLC for Practicing Scientists - Fundamentals,” explaining the technique.

This week, we’re proud to present a three-part series of segments from the first episode and encourage our readership to access the entire series, which offers a free, comprehensive, educational overview of the technique for scientists at any career stage seeking a refresher. We’re sure that, regardless of where you stand concerning experience with this technique, you’ll pick up pointers to hone your skills.

In this installment, Dong presents a brief history of the technique.

To access the entire “HPLC for Practicing Scientists” series, visit https://www.youtube.com/channel/UCEbs_GMZwMsKg-CqzzbfSkA

Review Michael’s “Perspectives in HPLC” columns in 2024-2025:
The Good, the Bad, and the Ugly of HPLC in Pharmaceutical Analysis
HPLC is indispensable in pharmaceutical analysis and quality control despite some notable shortcomings in laboratory testing and processes to ensure regulatory compliance.

New HPLC, MS, and CDS Products from 2024–2025: A Brief Review
This review highlights the high-performance liquid chromatography (HPLC), mass spectrometry (MS), chromatography data systems (CDS), and related products that were introduced from 2024 to 2025, summarizing the technical specifications of each product and highlighting significant user benefits.

Sample Preparation of Drug Substances and Products in Regulated Testing: A Primer
Michael discusses sample preparation (SP) techniques for small-molecule drug substances and solid dosage forms.

A Well-Written Analytical Procedure for Regulated HPLC Testing
Michael describes the content of a well-written analytical procedure for regulated high-performance liquid chromatography (HPLC) testing. A stability-indicating HPLC assay for a drug product illustrates the required components for regulatory compliance, including additional parameters to expedite a laboratory analyst’s execution.

Additional columns can be accessed here.