HPLC Troubleshooting Guide to Cycling Baselines and Pressure Fluctuations


HPLC troubleshooting guide to cycling baselines and pressure fluctuations from LCGC's ChromAcademy.



The Problem

A short term cycling baseline is seen along with pressure fluctuations (Figure 1).

Figure 1: Shor-term cycling baseline.


The CHROMacademy HPLC Troubleshooter

Both chromatographic (up to three) and instrument symptoms (up to two) can be selected at one time. The chromatographic symptom which is being seen is a short term cycling baseline along with pressure fluctuations, which is instrument related (Figure 2). Each option has a helpful rollover which illustrates the problem; this can be useful if you are not entirely sure what problem you are seeing.



Figure 2: Input of chromatographic (top) and instrument (bottom) symptoms into the CHROMacademy HPLC Troubleshooter.

The complete article is available to CHROMacademy Lite and Premier members here >>

CHROMacademy Lite Membership is FREE and it only takes two minutes to register.

With a Lite Membership you are given access to:

  • This month's webcast & tutorial

  • Selected eLearning modules

  • Featured CHROMacademy Content

  • The CHROMacademy forum

Test drive CHROMacademy and Check out more great content available FREE to our Lite members »

Related Content