Phospholipid removal proves to yield superior results as compared to a traditional protein precipitation step when analyzing a complex plasma matrix via LC–MS-MS. The results displayed a decrease in ion suppression, increased analyte sensitivity, and an improvement in column lifetime.
Analyze Charge Variants in Antibody-Based Therapeutics Using Cation Exchange Chromatography
July 18th 2024This application note discusses the latest development on high performance cation exchange bio-separation columns for determining charge variants in protein-based biotherapeutics, as well as the strategy on method development.