Flow rate is a common method parameter for most adsorptive LC methods. However, SEC is unique in that lower flow rates often yield improvements in separation, and resolution can improve considerably simply by running at a lower linear velocity. This application note demonstrates this approach for the aggregate analysis of NIST mAb, a common standard protein.
Pick Your Poison: Isolation of Paclitaxel (Octt 2024)
October 4th 2024The diterpenoid, paclitaxel, which was identified as a potent chemotherapy agent for breast and ovarian cancer originates from the Pacific Yew tree. The isolation of paclitaxel from its major impurities is shown with the use of Hamilton’s PRP-1 (5 µm) HPLC column.