The Increasing Importance of Temperature in Liquid Chromatography

March 5, 2012

E-Separation Solutions

This Tuesday morning session will be presided over by Matthew R. Linford of Brigham Young University (Provo, Utah), who also arranged the session.

Session 960

Room 308C

This Tuesday morning session will be presided over by Matthew R. Linford of Brigham Young University (Provo, Utah), who also arranged the session.

The session will open with introductory remarks from Linford. The first presentation in the session, “The Role of Temperature in HPLC Selectivity,” will be given by LCGC “LC Troubleshooting” columnist and editorial advisory board member John Dolan of LC Resources. Dolan will discuss the power of temperature in controlling high performance liquid chromatography (HPLC) selectivity and will look at how to avoid problems encountered if the temperature is improperly controlled.

Next, Linford will present a talk titled “Van’t Hoff Analysis in Liquid Chromatography Applied to Thermally Stable Materials.” His presentation will describe experiments performed at elevated temperature and high pH with a new thermally stable C18 stationary phase.

The next presentation, to be given by LCGC editorial advisory board member Peter Carr of the University of Minnesota, is titled “High Speed Two-Dimensional LC at Elevated Temperatures.” His talk will discuss the history and theory of high-temperature LC as a way to increase separation speed.

Frank L. Dohrman of The Pennsylvania State University (University Park, Pennsylvania) will then present a talk titled “Coupling the Hydrophobic Subtraction Model with Organic Modifiers and Elevated Temperature for Optimization of Selectivity and Efficiency.” His presentation will discuss the hydrophobic subtraction model and how it relates to retention mechanisms in HPLC at elevated temperatures.

The final presentation in the session will be given by a third LCGC editorial advisory board member, Pat Sandra of the Research Institute for Chromatography (Kortrijk, Belgium) and is titled “The Role of Temperature in HPLC Efficiency and Speed.” Sandra’s talk will discuss his laboratory’s research on optimizing separation speed and efficiency by selecting the correct temperature in reversed-phase LC, HILIC, and enhanced-fluidity LC.

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