Monday Afternoon Session Preview: Clinical Chemistry: Dried Blood Spot Analysis

June 5, 2013

E-Separation Solutions

Monday afternoon?s six presentations will focus on dried blood spot analysis, including the development of enzyme assays based on tandem mass spectrometry for the detection of lysosomal storage disorders; analysis of hemoglobin peptides in tryptic digests of dried-blood spot extracts; in-paper dried blood spot enzyme assays to determine plasma enzyme activities; the determination of neonatal paraben exposure; the introduction of a ?detergent-assisted elution? to improve analyte elution efficiency in dried matrix spot bioanalytical assays by UHPLC?MS-MS; and a method to quantify peptides from insulin-like growth factor-1 and its synthetic analogues in dried blood spots by LC?MS-MS.

Monday, 2:30 - 4:30 PM, Ballroom B

Monday afternoon’s six presentations will focus on dried blood spot analysis, including the development of enzyme assays based on tandem mass spectrometry for the detection of lysosomal storage disorders; analysis of hemoglobin peptides in tryptic digests of dried-blood spot extracts; in-paper dried blood spot enzyme assays to determine plasma enzyme activities; the determination of neonatal paraben exposure; the introduction of a “detergent-assisted elution” to improve analyte elution efficiency in dried matrix spot bioanalytical assays by UHPLC–MS-MS; and a method to quantify peptides from insulin-like growth factor-1 and its synthetic analogues in dried blood spots by LC–MS-MS.

The first presenter will be Mariana Barcenas of the Department of Chemistry, University of Washington, Seattle, Washington. Barcenas, whose talk is titled “Multiplex Newborn Screening of Lysosomal Storage Diseases Using Flow Injection Tandem Mass Spectrometry,” will discuss current efforts that are focused on developing a multiplex assay to diagnose six disorders–Krabbe, Gaucher, Nieman-Pick, Fabry, Pompe, and Mucopolysaccharidosis I–using flow injection analysis.

Christopher A. Haynes, of the Centers for Disease Control and Prevention, in Atlanta, Georgia, will present next. His talk, titled “HPLC-ESI-MS-MS Analysis of Hemoglobin Peptides in Tryptic Digests of Dried-Blood Spot Extracts Detects HbS, HbC, HbE, HbO-Arab and HbG-Philadelphia mutations,” will describe a method that provided greater than 90% coverage of the β-globin, α-globin, and γ-globin amino acid sequences, which are components of HbA and HbF (normal adult and fetal hemoglobins, respectively).

Following Haynes, Eszter Szabo, from Eötvös Lóránd University, Budapest, Hungary, will give a talk titled “In-Paper Dried Blood Spot Enzyme Assays for the Reliable Determination of Plasma Enzyme Activities.” Haynes will discuss robust, quantitative mass spectrometry methods that were developed for determination of biotinidase and GGT activities in pre-treated dried blood spot samples.

Up next will be Shirish Yakkundi, from Queen’s University Belfast, Belfast, UK, presenting “Application of LC–MS-MS to Determine the Exposure to Methyl and Propyl Parabens in Preterm Neonates Using Dried Blood Spot.” Yakkundi will explain how dried blood spot assay of parabens will enable the prediction of neonatal paraben exposure.

Naiyu Zheng will follow Yakkundi, with a presentation titled “Detergent-Assisted Elution: Method Optimization to Improve Analyte Elution and Assay Performance for Dried Matrix Spots by UHPLC–MS-MS.” Zheng will demonstrate how detergent-assisted elution could be useful in dried blood spots or other dried matrix spots assays for other analytes.

The final presentation will be give by Holly Cox, of the Sports Medicine Research and Testing Laboratory, Salt Lake City, Utah. Cox’s talk, “Sensitive Quantification of Insulin-Like Growth Factor-1 and Its Synthetic Analogues in Dried Blood Spots,” will focus on a method that measures two trypsin peptides of endogenous IGF-1.

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