Multiresidue Antibiotic Analysis
Scientists from the USDA';s Eastern Regional Research Center (Wyndmoor, Pennsylvania) analyzed 11 beta-lactam antibiotics in bovine kidney using a sample preparation method consisting of an acetonitrile-water extraction followed by a dispersive solid-phase extraction with a C18 sorbent, extract concentration, and filtration with syringeless filter vials.
Scientists from the USDA's Eastern Regional Research Center (Wyndmoor, Pennsylvania) analyzed 11 beta-lactam antibiotics in bovine kidney using a sample preparation method consisting of an acetonitrile-water extraction followed by a dispersive solid-phase extraction with a C18 sorbent, extract concentration, and filtration with syringeless filter vials. The final filtered extracts were analyzed by liquid chromatography-tandem mass spectrometry. The antibiotics included amoxicillin, ampicillin, cefazolin, cephalexin, cloxacillin, DCCD, deacetylcephapirin, dicloxacillin, nafcillin, oxacillin, and penicillin G.
Study Explores Thin-Film Extraction of Biogenic Amines via HPLC-MS/MS
March 27th 2025Scientists from Tabriz University and the University of Tabriz explored cellulose acetate-UiO-66-COOH as an affordable coating sorbent for thin film extraction of biogenic amines from cheese and alcohol-free beverages using HPLC-MS/MS.
Multi-Step Preparative LC–MS Workflow for Peptide Purification
March 21st 2025This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.
