Need to Achieve Confident Oligonucleotide Characterization and Purification?

Learn how to confidently analyze and purify oligonucleotides with the latest techniques and methods. Join our webinar and interact with our experts. Register now and save your spot!

Register Free: https://www.chromatographyonline.com/lcgc_w/confident

Event Overview:

Oligonucleotides have grown to represent an increasingly important class of therapeutic modalities. Most oligonucleotides (OGNs) are chemically synthesized using a 4-step cyclic process of the growing oligomer. Thus, the complete characterization and purification of the desired OGN is necessary, before moving to the next step. In this webinar presentation, we will discuss how you can confidently analyze the OGN purity and the related impurities, and determine its sequence, followed by the efficient purification of OGNs with respect to lengths of OGNs and possible modifications.

Key Learning Objectives:

• Oligonucleotide identity, impurities analysis and sequence determination using LC/Q-TOF and BioConfirm 12.0 target plus impurities (TPI) and sequence confirmation workflows
• Ion-pairing free method using LC/TOF and HILIC enabling accurate and repeatable analysis of diverse oligonucleotides
• Improved purification of OGNs by combining improved instrumental setup with modern IP-RPLC chromatography as well as fraction analysis with both LC-UV and LC-MS detection

Who Should Attend:

• Oligonucleotide Analytical Method Development Scientists
• Oligonucleotide Purification method Development Scientists
• Oligonucleotide Process Development Scientists
• Oligonucleotide QC Scientists
• Oligonucleotide CDMO Labs

Speakers:

Olof Stålberg, Ph.D
Senior Development Engineer
Qiagen DNA synthesis (QDS)
ästerås, Sweden

Olof Stålberg is currently senior development engineer at Qiagen DNA Synthesis in Västerås, involved in separation science, validation, and development of technical and scientific improvements. He has extensive cross-functional and global working experience, and is a former project leader and coordinator for technical and pharmaceutical projects at the former Astrazeneca (AZ) R&D in Södertälje, Sweden. Dr. Stålberg studied at the Biopharmaceutical Science Center at Leiden, Linköping University, Karlstad University and ultimately earned his doctorate from Uppsala University.

Yulan Bian
R&D Scientist
Agilent Technologies

Yulan Bian holds a Master’s degree in Biomedical Engineering with over 15 years of experience in analytical instrumentation. She is currently working as an R&D scientist at Agilent, where she specializes in protein and oligonucleotide analysis. Prior to joining Agilent, she worked as a Research Assistant at Nanyang Technological University Singapore, where she focused on the purification and characterization of polypeptides.

Lee Bertram
Application Development Engineer
Agilent Technologies

Lee is an application scientist at Agilent with a focus on development of methods for SQ, QQQ and QTOF within the pharmaceutical products. He has over 10 years of method development experience ranging from large/small molecule pharmaceutics to cellular metabolomics method development.

Register Free: https://www.chromatographyonline.com/lcgc_w/confident