Oral Session: CE Separation Strategies

Session 860

Room 406, 8:00 a.m.

This Tuesday morning session will be presided over by Mary Ellen McNally (DuPont Crop Protection).

The session will begin with a presentation by Bingchuan Wei of Purdue University (West Lafayette, Indiana) titled “Submicrometer Plate Heights for Proteins Using CEC: Mechanism and Applications.” Wei’s discussion of capillary electrochromatography separations in silica colloidal crystals modified with C4 examines the extremely small plate heights of the technique and the mechanism enabling such small plate heights.

The next presentation in the session is titled “Optimizing a Short-end Electrophoretically Mediated Micro-analysis (EMMA) Assay for Creatinine” and will be delivered by Aravinda Seneviratne of Bucknell University (Lewisburg, Pennsylvania). Seneviratne will discuss the combination of electrophoretically mediated microanalysis with short-end injection to improve the in-capillary Jaffe assay for creatinine.

Qing Feng of the University of Texas at Arlington will present the next talk, titled “Separation of Trivalent Anions by CE Using a Phosphonium-based Tetracationic Reagent.” This presentation will cover a capillary electrophoresis (CE) method using a tetracationic phosphinium–based bromide background electrolyte additive to aid in the separation of singly charged inorganic and small organic anions.

The next presentation will be given by Christopher R. Harrison of San Diego State University (San Diego, California) and is titled “An Amphipathic Polymer as a Buffer Additive for Capillary Zone Electrophoretic Separations of Proteins.” Amphipathic compounds possess both polar and nonpolar functional groups, and one used in this study prevented protein adsorption to the capillary surface and modified the electroosmotic flow.

Following a 15-min recess, Brooke Koshel of Purdue University will present a talk titled “Capillary Isoelectric Focusing of Proteins with Field-Free Remobilization.” Koshel will discuss the use of capillaries packed with nonporous particles modified with polyacrylamide to achieve separations in minutes.

Joseph T. Maloy of Seton Hall University (South Orange, New Jersey) is scheduled to present the next talk, titled “Finite-Difference Simulation of Adsorption Effects in Partition Chromatography.” This presentation will discuss a software approach for modeling surface adsorption effects.

Christopher R. Harrison of San Diego State University is slated to present a second talk, “Varied Electroosmotic Flows from Mixed Onium Buffer Additives.” The authors investigated the influence of various onium compounds (such as ammonium and phosphonium compounds) on the magnitude of the electroosmotic flow.

The final presentation in the session is scheduled to be given by Constantina Panayioti Kapnissi-Christodoulou of the University of Cyprus (Nicosia, Cyprus) and is titled “Comparison of MEKC and OT-SEC for the Separation of Acetylcholinesterase Inhibitors — Optimization of LOD and LOQ Using CE–MS.” This study evaluated chromatographic parameters such as the buffer, the SDS and anionic polymer concentration, the pH, the background electrolyte composition, the number of bilayers, and the deposition time of the polymers.