μPAC™ column robustness in bottom-up proteomics

Polysome profiling is a method for analysing the translational status of cells. This AppNote describes a simple FPLC system for polysome fractionation.

Pfizer streamlined their LC/UV/MS data processing by adopting a semi-automated workflow with MS Workbook Suite, enabling faster, more consistent analysis and cutting process

Organic solvent, pH, salt concentration, and gradient mixing rates influence HIC separations. Optimizing these factors allows for effective separation of mAbs and ADCs.

Analytical scientists are faced with the task of finding the right column from an almost unmanageable range of products. This paper focuses on columns that enable protein analysis under native conditions through size exclusion, hydrophobic interaction, and ion exchange chromatography. It will highlight the different column characteristics—pore size, particle size, base matrices, column dimensions, ligands—and which questions will help decide which columns to use.

Not all chromatography resins are created equal. Off-the-shelf chromatography resins might not always meet the rigorous purification requirements of biopharmaceutical manufacturing. Custom bonded silica from Grace can address a wide range of separation challenges, leading to real performance improvements. Discover more about the latest innovations in chromatography silica from Grace, including VYDAC® and DAVISIL®.