Rapid Screening and Quantitation of 534 Pesticides From Food By LC–MS-MS

The Application Notebook

The Application Notebook, The Application Notebook-09-01-2011, Volume 0, Issue 0

With more than 800 pesticides currently in use there is growing concern by government agencies about the effect of pesticide exposure on human health. While analytical techniques like GC or GC–MS have traditionally been used for pesticide analysis, LC–MS-MS has become the technique of choice due to speed, increased sensitivity, and reduced sample preparation. The following describes an LC–MS-MS method for screening of 534 pesticides in food products.

With more than 800 pesticides currently in use there is growing concern by government agencies about the effect of pesticide exposure on human health. While analytical techniques like GC or GC–MS have traditionally been used for pesticide analysis, LC–MS-MS has become the technique of choice due to speed, increased sensitivity, and reduced sample preparation. The following describes an LC–MS-MS method for screening of 534 pesticides in food products.

Figure 1: MRM ion traces for pesticides at 10 ng/mL.

Experimental Conditions

Sample preparation from vegetable, nut, and citrus plant materials was done using a standard QuEChERS cleanup procedure and chromatographic separation of the pesticides was achieved using a Phenomenex® Synergi™ Fusion-RP (polar embedded C18) HST 2.5 μm, 50 × 2 mm HPLC column. The method for the AB Sciex 3200 QTRAP® LC–MS-MS system was downloaded from the iMethod™ Applications store (www.absciex.com/iMethods) and contained all the LC methods, optimized MS settings for each analyte, processing methods, reporting templates, complete method documentation, and a Pesticide Standard Kit, iDQuant™ that contained 204 compounds distributed in 10 vials. The MS-MS library database of over 601 pesticides was also downloaded, which enabled identification of unknown peaks based on the dual MRM and MS-MS functionality of the QTRAP System.

Table I: Representative recoveries, signal-to-noise ratios and estimated detection limits for selected pesticides spiked at 25 ng/mL in a cucumber matrix

Results

534 pesticides were screened in less than 12 min. Table I provides representative recoveries, signal-to-noise ratios, and estimated detection limits for selected pesticides spiked at 25 ng/mL in a cucumber matrix. For a full list of recoveries and performance, visit www.absciex.com/iMethods. The final method was verified at different laboratory facilities to ensure the robustness of the method.

Phenomenex Inc.

411 Madrid Avenue, Torrance, CA 90501

tel. (310) 212-0555, fax (310) 328-7768

Website: www.phenomenex.com