SFC can provide solutions for challenging separations beyond traditional normal- and reverse-phase HPLC. But to get the best results, you need to select the right columns. This eBook examines column options for accurately and efficiently separating a variety of pharmaceutical compounds using SFC. Topics include SFC column fundamentals and considerations, selecting SFC columns, and columns for SFC chiral and achiral analysis.
Ultrahigh-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) tracks blood metabolite changes after repetitive transcranial magnetic stimulation (rTMS)-cognitive training therapy.
Revisiting insertion distance for methanol and methylene chloride in GC analysis and confirming that insertion depth strongly affects response and reproducibility.
A novel SFC–QTOF-MS method accurately detected 10 drugs in oral fluid, matching LC–MS/MS in 83 DUID cases while reducing run time by 42% and organic solvent use by 81%.
Supercritical fluid extraction (SFE) and high-pressure liquid chromatography (HPLC) team up to recover olive waste antioxidants. Marina Russo of the University of Messina (Italy) discusses the details.
Waters' Erin Chambers says combining column chemistry and particle-physics innovation cut a GLP-1 impurity profiling workflow from three hours to under one.