Shimadzu Corporation

Per- and polyfluoroalkyl substances (PFAS) are a diverse group of over 4000 organic fluorinated compounds, which have been widely used in industry since the 1950s. Due to their widespread presence and potential health risks, accurate PFAS analysis is essential to monitor contamination, ensure regulatory compliance, and protect human health and the environment. In this eBook, we explore our range of chromatography and mass spectrometry (MS) solutions for detecting very low levels of PFAS in matrices such as drinking water, wastewater, ambient air, foodstuffs, and consumer products. Plus, learn through a series of case studies how our LC-MS/MS systems and other analytical instruments (GCMS, CIC, QTOF…) meet and often exceed performance criteria included in EPA and AOAC methods for PFAS analysis – generating accurate results with fast turnaround times that enhance lab productivity.

Innovation and quality drive every stage of beverage production—from early R&D to the final sip. Advanced GCMS and Molecular Spectroscopy solutions help producers ensure excellence across the entire beverage life cycle, from R&D and regulation/safety compliance to QA/QC, shelf life, and packaging applications. Whether crafting coffee, soft drinks, or alcoholic beverages, this infographic describes how Shimadzu’s technology elevates the beverage-making process to ensure reliable, consistent, quality products.

In this case study, an improvement in oligonucleotide peak shape is shown by suppressing the sample solvent effect through simultaneous injection with a desired solvent while using dedicated method development software to comprehensively evaluate co-injection solvent types and volumes to enable a more efficient workflow.

The article describes a comprehensive automated prep purification workflow targeting semaglutide, a GLP-1 receptor agonist, using dedicated method development software to automatically optimize a gradient profile and search for conditions that meet the specified separation criteria. This was followed by scale-up to purification scale and streamlined confirmation of the purities of collected fractions using benchtop MALDI-TOF MS.

This article describes how to efficiently achieve the optimal peak separation for charge variants of a monoclonal antibody using pH gradient ion-exchange chromatography and LabSolutions MD, dedicated software that can automate the entire method development workflow, including the generation of an analysis schedule, mobile phase preparation, and data processing.

This article describes the efficient development of robust analytical methods based on Analytical Quality by Design (AQbD) using dedicated LabSolutions MD software, which supports each phase of the process – screening, optimization, and validation – using functions such as experimental design, building of design space by automatic Peak Tracking, and robustness evaluation.

This article describes how dedicated software for supporting method development, in conjunction with single quad LCMS and inert UHPLC, can improve the overall method development workflow and efficiently achieve the optimal separation of oligonucleotides and related impurities.

This case study illustrates the streamlining of SFC method development using a mixture of six small molecule pharmaceuticals as a model sample, from automatic screening of columns and modifiers to design space evaluation for optimal conditions.

This poster presents an example of the analysis of all 17 explosives listed in EPA 8330B using atmospheric pressure chemical ionization liquid chromatography mass spectrometry (APCI-LC-MS/MS), which allowed for more sensitive and selective analysis than PDA detection. In addition, MRM analysis provides an extra layer of confirmation based on the unique fragmentation of each compound.

This poster presents a comprehensive, multi-approach data processing method for the analysis of non-targeted PFAS in both positive and negative ionization modes. Results show this three-pronged approach gave comprehensive identifications without excess false positives.

This poster shows the development and validation of a fully automated sample preparation and subsequent LC-MS/MS with trap-and-elute chromatography assay analyzing 25-hydroxyvitamin-D2 and D3. With this automated platform, technicians can significantly reduce their active time in the sample preparation workflow, focusing primarily on sample registration and results review.

EPA Method 1633A is currently the only validated multi-lab method for PFAS detection in leachate. This poster demonstrates the effectiveness of a system to provide robust, stable performance over 154 injections and 37 hours of continuous PFAS analysis, making it ideal for routine monitoring of complex leachate matrices.

Webinar Date/Time: Wed, Nov 6, 2024 2:00 PM EST

Due to their ability to tackle targets that were considered undruggable, oligonucleotides represent a key cornerstone of the future of personalized medicine. This infographic presents the different types of therapeutic oligonucleotides and highlights some innovative solutions for their analysis.

Discover how the Brevis™ GC-2050 is Leading the Way in Compact Gas Chromatography

Webinar Date/Time: Wed, Aug 7, 2024 11:00 AM EDT

Cannabinoid separation can be difficult with the presence of coeluting isomers. This application shows how a combination of LabSolutions MD (Method Development) software and powerful i-PDeA technology can help determine the optimal method for cannabinoid separation and deconvolute remaining co-eluting peaks after optimization.

This application showcases how a compact, affordable single quad LCMS can be easily integrated into existing laboratories for measurement of PFAS in diverse samples where adherence to regulated methods (e.g., EPA 533, 537.1, and 1633) is not required, or samples where expected concentrations are larger than sub ppb levels.

This application news presents instrument operating conditions and instrument method performance statistics, including method linearity, accuracy, and precision, for priority pollutant pesticides and PCB congeners. The method validated in this application acted as guidance in developing ASTM D8543 Test Method for Determination of OCPs and PCBs in Aqueous Solution by a GC/MS/MS which is intended for proposal to EPA as an alternative test procedure (ATP) for EPA 608.3

Webinar Date/Time: Thu, Jan 25, 2024 2:00 PM EST

Today’s SFC systems are widely accepted for analytical and preparative separation of chiral and achiral compounds related to pharmaceutical development. Shimadzu enhances the user experience by delivering a highly effective integration of analytical and prep SFC supported by a unified software platform. Learn how these solutions can be used for rapid screening of multiple analytical conditions, simplified method development, and purification at laboratory scale.

Whether you're an experienced user of SFC technology or just getting into it, this FAQ guide offers a wealth of knowledge to enhance your efficiency, troubleshoot common issues, and make the most of this dynamic tool. Learn about a wide range of topics, including hardware insights, advantages of SFC, applications, and column screening and matrix effects.

SFC can provide solutions for challenging separations beyond traditional normal- and reverse-phase HPLC. But to get the best results, you need to select the right columns. This eBook examines column options for accurately and efficiently separating a variety of pharmaceutical compounds using SFC. Topics include SFC column fundamentals and considerations, selecting SFC columns, and columns for SFC chiral and achiral analysis.

The Nexera UC UHPLC/SFC switching system combines both UHPLC and SFC analysis modes in a single system, allowing you to easily switch between the two techniques in as few as ten minutes without reconnecting flow lines. So, you get the added resolution of UHPLC and the added selectivity of SFC to quickly develop separations without compromising chromatographic accuracy. Learn about five key benefits.

This poster demonstrates the utilization of a highly sensitive and accurate LC-MS/MS method for the quantitation of select endogenous steroids using dried blood spots (DBS) serum separator cards.

The work illustrates a single LCMS method for the analysis of AZBT and AMBBT in five sartan drug substances, with full separation of all sartans and azido impurities achieved in only 8.5 minutes.

LabSolutions DB/CS software offers powerful support for ensuring compliance with data integrity requirements for all types of analytical instruments, and can be optimized for a wide range of laboratory sizes and operating styles. Download this file to learn more.

In this poster, the 40 PFAS from EPA method 1633 were used to generate a library from a single mixture, and then were confirmed to function for targeted library screening and untargeted identification in groundwater.

This work demonstrates the benefits of adding a compact single quad LCMS into an existing workflow for API screening and analysis.