Semi-Micro Columns for Size-Exclusion Chromatography in Organic Solvent Systems: TSK-GEL SuperMultiporeHZ Series Columns

September 1, 2008
Xavier Villarreal

The Application Notebook

The Application Notebook, The Application Notebook-09-01-2008, Volume 0, Issue 0

In size exclusion chromatography, obtaining calibration curves over a wide range of molecular weights is a difficulty investigators often encounter when analyzing polymers with a broad molar mass distribution. To overcome this problem two procedures are typically used. One option is to use multiple columns of different pore sizes linked together in series. A second is to use a column packed with a mixed bed resin of different pore sizes at an optimized mix ratio. However, problems can occur with both of these methods, which include distortion of the chromatogram or deviations between the actual calibration curve and the calibration curve approximated from data obtained from the molecular weight standards.

Xavier Villarreal, Ph.D., Tosoh Bioscience LLC

In size exclusion chromatography, obtaining calibration curves over a wide range of molecular weights is a difficulty investigators often encounter when analyzing polymers with a broad molar mass distribution. To overcome this problem two procedures are typically used. One option is to use multiple columns of different pore sizes linked together in series. A second is to use a column packed with a mixed bed resin of different pore sizes at an optimized mix ratio. However, problems can occur with both of these methods, which include distortion of the chromatogram or deviations between the actual calibration curve and the calibration curve approximated from data obtained from the molecular weight standards.

Figure 1

As is shown in Figure 1, a novel approach to solve this problem was developed by Tosoh scientists and is incorporated in TSK-GEL SuperMultiporeHZ Series columns. Using proprietary multi-pore particle synthesis technology, packing material composed of monodisperse particles are synthesized in which a wide range of pore sizes are contained within a single particle. The TSK-GEL SuperMultiporeHZ Series product line further improves performance by reducing column dimensions to the semi-micro level (4.6mm ID × 15cm), which cuts down on solvent consumption costs by one-sixth and running times by one-half.

Table I

Results

Figure 1 compares the separation of oligomers in poly(tetramethylene ether glycol) (PTMEG 650) of four conventional mixed bed GPC columns (A) to the TSKgel SuperMultipore-HZ-N column (B). The TSKgel SuperMultiporeHZ-N column (B) provides the same or higher resolution at a much shorter analysis time than multiple columns linked together.

Table II shows the average molecular weight of silicone resin analyzed on several different lots of TSK-GEL SuperMultipore-HZ-N columns. The data reveals that there is very little difference between lots.

Table II

Conclusion

TSK-GEL SuperMultiporeHZ Series size exclusion columns represent an innovative type of semi-micro GPC column. These columns have an extended linear MW range with superior reproducibility. Using these products lowers solvent consumption and disposal expenses while offering higher-throughput capabilities. There are three columns available within the TSK-GEL SuperMultiporeHZ Series, each with a different particle size, separation range, and exclusion limit. These columns can separate and characterize polymers within a wide molecular mass range.

Tosoh Bioscience LLC

156 Keystone Drive, Montgomeryville, PA 18936

Tel. 215-283-5000 / 800-366-4875

Fax: 215-283-5035

www.tosohbioscience.com