Separations of Intact IgGs using non-porous, 2 µm particle, C18 Presto column, with a view towards ADC analysis

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LCGC North America

Intact and reduced IgGs have been successfully retained and separated using a non-porous column. Potential applications of Presto columns for a single-injection separation of free drug, antibody, and antibody-drug complexes (ADCs) is also discussed.