Slalom Chromatography Revisited (Part 2); An Interview with Fabrice Gritti

LCGC International spoke to Fabrice Gritti, consultant scientist at Waters Corporation, about the history of slalom chromatography (SC) and why he decided that the technique was worth re-investigating. The potential benefits of SC, according to Gritti, include identifying RNA impurities when manufacturing mRNA therapeutics, and assisting in the development of new gene and cell therapies, and other biopharmaceutical applications.

• How does slalom chromatography help to identify impurities in RNA used for making mrNA therapeutics?
• Are there any other potential benefits for slalom chromatography in biopharmaceutical analysis?
• How could SC assist in the development of new gene and cell therapies?
• Could slalom chromatography possibly be used in any other applications?

Biography
Fabrice G. Gritti received a PhD in chemistry and physics of condensed matter from the University of Bordeaux I (France) in 2001. He worked as a research scientist at the University of Tennessee (Knoxville, Tennessee, USA) from 2002–2014 in the research group of the late Professor Georges Guiochon. He joined Waters Corporation in 2015 where he is currently a consulting scientist.

References
(1) Gritti, F. Ultra-High Pressure Slalom Chromatography: Application to the Characterization of Large DNA and RNA Samples Relevant in Cell and Gene Therapy. J. Chromatogr. A 2024, 1781, 465487. DOI: 10.1016/j.chroma.2024.465487.

(2) Gritti, F. Theore. Theoretical Predictions to Facilitate the Method Development in Slalom Chromatography for the Separation of Large DNA Molecules. J. Chromatogr. A 2024, 1706, 465379. DOI: 10.1016/j.chroma.2024.465379