News|Articles|February 21, 2024

Targeted Analysis of Phosphorylated Metabolites

Author(s)HILICON AB

Crucial phosphorylated compounds can be analyzed via polymeric iHILIC-(P) Classic HILIC columns and MS. This is applicable for targeted and non-targeted metabolomics.


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Figure 2. Split injection used to measure triplicate test of MTBE, hexane, o-xylene, and 1-methylnaphthalene comparing different solvents (methanol, methylene chloride) and column insertion distances starting with 0.5 mm, 5.0 mm, 10.0 mm, 15 mm, and 20.0 mm. Methanol had the best performance at the 5.0 mm insertion distance and methylene chloride looked slightly better at the 0.5 mm. We would still recommend not going below the 5.0 mm manufacturer recommended insertion distance.
Revisiting insertion distance for methanol and methylene chloride in GC analysis and confirming that insertion depth strongly affects response and reproducibility.