Tips & Tricks: GPC/SEC Peak Identification in GPC/SEC

Analyzing a complex mixture of unknown macromolecules and low molar mass compounds is often challenging in GPC/SEC. A disadvantage of the most commonly used detector, the very universal but unspecific refractive index (RI) detector, is that this detector only provides concentration information and no information about the chemistry. In addition, various positive and negative peaks besides the pure sample peaks in a GPC/SEC run are normal.