News|Articles|November 20, 2023

Using SFC to Improve Separation Workflows for Drug Analysis and Purification

Today’s SFC systems are widely accepted for analytical and preparative separation of chiral and achiral compounds related to pharmaceutical development. Shimadzu enhances the user experience by delivering a highly effective integration of analytical and prep SFC supported by a unified software platform. Learn how these solutions can be used for rapid screening of multiple analytical conditions, simplified method development, and purification at laboratory scale.


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Figure 2. Split injection used to measure triplicate test of MTBE, hexane, o-xylene, and 1-methylnaphthalene comparing different solvents (methanol, methylene chloride) and column insertion distances starting with 0.5 mm, 5.0 mm, 10.0 mm, 15 mm, and 20.0 mm. Methanol had the best performance at the 5.0 mm insertion distance and methylene chloride looked slightly better at the 0.5 mm. We would still recommend not going below the 5.0 mm manufacturer recommended insertion distance.
Revisiting insertion distance for methanol and methylene chloride in GC analysis and confirming that insertion depth strongly affects response and reproducibility.
Basel, Switzerland. Old town with red stone Munster cathedral on the Rhine river. © SCStock - stock.adobe.com
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