LCGC North America
In this article, the authors review recent developments in the research of whole-column-imaging detection for capillary electrophoresis (CE). Whole-column-imaging detection was developed for capillary isoelectric focusing, for which it proved to be an ideal detector. Several whole-column-imaging detectors -- including refractive index gradient imaging, UV-absorption imaging, and fluorescence imaging detectors -- have been studied. The capillary isoelectric focusing UV-absorption imaging technique even has been commercialized. The development of whole-column-imaging detection itself facilitates CE studies in many directions such as in electrophoretic dynamics within narrow channels, new separation modes, and two-dimensional separations. Whole-column-imaging detection also finds application in capillary zone electrophoresis.
Essentials of LC Troubleshooting, Part VII: How Wide Should Those Peaks Be? (Large Molecule Edition)
June 5th 2025In the latest edition of "LC Troubleshooting", Dwight Stoll explores troubleshooting tips for liquid chromatography, particularly focusing on peak width challenges in large molecule separations and effective solutions.