AMT

Separations of Oligonucleotides using HALO 1000 Å OLIGO C18 show improved peak capacity, narrower peaks, and lower backpressure vs. 300 Å FPP columns.

HALO 1000 Å OLIGO C18 enables high-resolution impurity analysis of 90-mer ssDNA, outperforming competitors in peak capacity and separation efficiency.

Larger pore columns improve efficiency and reduce band dispersion in oligonucleotide separations-especially at high flow rates and longer chain lengths.

Longer columns paired with scaled gradients enhance oligonucleotide resolution especially for complex mixtures up to 100 nucleotides in length.

Discover how HALO® OLIGO C18 columns improve DNA/RNA separation with optimized ion pairing, pH control, and particle morphology for faster, sharper results.

Webinar Date/Time: Tuesday, December 2, 2025 8am PST | 11am EST | 4pm GMT | 5pm CET

Harry Ritchie, PhD, Director of Business and Market Development at Advanced Materials Technology, shares how crucial sustainability has become in the field of chromatography, and provides helpful advice for labs looking to meet sustainability initiatives.

Peter Pellegrinelli, Applications Specialist at Advanced Materials Technology (AMT) explains the complexity of oligonucleotide separations due to the unique chemical properties of these molecules. Issues such as varying length, sequence complexity, and hydrophilic-hydrophobic characteristics make efficient separations difficult. Separation scientists are addressing these challenges by modifying mobile phase compositions, using varying ion-pairing reagents, and exploring alternative separation modes like HILIC and ion-exchange chromatography. Due to these complexities, AMT has introduced the HALO® OLIGO column, which offers high-resolution, fast separations through its innovative Fused-Core® technology and high pH stability. Alongside explaining the new column, Peter looks to the future of these separations and what is next to come.

Peter Pellegrinelli, Applications Specialist at Advanced Materials Technology (AMT) explains the complexity of oligonucleotide separations due to the unique chemical properties of these molecules. Issues such as varying length, sequence complexity, and hydrophilic-hydrophobic characteristics make efficient separations difficult. Separation scientists are addressing these challenges by modifying mobile phase compositions, using varying ion-pairing reagents, and exploring alternative separation modes like HILIC and ion-exchange chromatography. Due to these complexities, AMT has introduced the HALO® OLIGO column, which offers high-resolution, fast separations through its innovative Fused-Core® technology and high pH stability. Alongside explaining the new column, Peter looks to the future of these separations and what is next to come.

In high-performance liquid chromatography (HPLC), it is common for bases and unreacted ionized silanols on silica-based columns to cause irreproducible retention, broad peaks, and peak tailing when working with basic analytes. David S. Bell, Lead Consultant at ASKkPrime LLC offers innovative HPLC strategies that can help mitigate such issues.

In high-performance liquid chromatography (HPLC), it is common for bases and unreacted ionized silanols on silica-based columns to cause irreproducible retention, broad peaks, and peak tailing when working with basic analytes. David S. Bell, Lead Consultant at ASKkPrime LLC offers innovative HPLC strategies that can help mitigate such issues.

Learn about the HALO® Elevate C18 column and how it enables more effective separation when it comes to basic compounds under high-pH conditions. Increased peak retention, improved peak shape, and enhanced ionization efficiency are all benefits explored in this paper.

Webinar Date/Time: Airing 1: Thursday, May 30, 2024 at 11am EDT | 12pm CDT | 8am PDT Airing 2: Friday, May 31, 2024 at 11am CST (Chinese Standard Time) | 12pm JST | 1pm AEST

The method conversion guidebook is an introduction to HALO® Fused-Core® technology, its benefits and how to take advantage of SPP for method conversion. This book will help you to facilitate converting conventional reversed-phase FPP separations to ultra-fast separations.

Built upon proven Fused-Core® technology for speed and efficiency, the HALO® PCS C18 is a positively charged surface chemistry designed to deliver improved peak shapes for basic compounds.

Following new USP guidelines for gradient method modernization enables both FPP and SPP methods to be improved for speed and solvent savings using HALO® Fused-Core® column technology while avoiding revalidation.

HPLC chromatographic methods can be greatly improved and modernized by simply reducing the column’s length and particle size. This not only saves time, but increases consumption and waste generation. By using modern HALO®Fused-Core® column technology, methods can be shortened thereby increasing laboratory efficiency while keeping back pressures under instrument pressure limitations.

HPLC and LC-MS separation materials and methodologies have evolved over the years to meet the challenges of the growing complexity of the separations themselves. In this Q&A, Stephanie Rosenberg, Director of Sales and Marketing discusses how utilizing the new HALO® positively charged surface columns impact the perforance of LC and LCMS separations for basic compounds.

This guide will discuss improved reversed phase biomolecule separation solutions for monoclonal antibodies, as well as chromatography columns used to develop an understanding of the protein backbone via peptide mapping. Glycosylation analysis using HILIC will also be discussed. Examples will highlight areas in the workflow where HALO® Fused-Core® columns with appropriate pore sizes and phases offer unique advantages.

Webinar Date/Time: Airing 1: Tuesday October 10, 2023 at 9am EDT | 8am CDT | 6am PDT Airing 2: Tuesday October 10, 2023 at 1pm EDT | 12pm CDT | 10am PDT

Webinar Date/Time: Thu, Jun 29, 2023 12:00 PM EDT

Webinar Date/Time: Wednesday, April 19, 2023

Wednesday, February 16, 2022 at 9am EST | 6am PST | 3pm CET | 2pm GMT Wednesday, February 16, 2022 at 2pm EST | 11am PST | 8pm CET | 7pm GMT Interested in increasing the sensitivity of your UHPLC separations and save solvent without having to invest in a specialized microflow system? Learn how to accomplish these goals with new 1.5 mm ID UHPLC columns packed with Fused-Core particles.

Learn the advantages of HILIC to measure and monitor glycosylation, deamidation, and oxidation, which are critical for the function and form of proteins and peptides.

To improve the chromatography of PFAS and other environmentally concerning chemicals, application-assured HPLC columns deliver high efficiency and robust analysis.