Chiara De Luca received her Ph.D. degree in chemical sciences at the University of Ferrara in 2021. She is currently a postdoc at the Department of Chemical, Pharmaceutical, and Agricultural Sciences at the University of Ferrara.
When van Deemter Goes Upside Down in Chiral Chromatography
May 2nd 2023The occurrence of convex-upward van Deemter curves is rare but not unusual in chiral liquid chromatography. In this work, this behaviour, experimentally observed for the more retained enantiomer of a chiral sulfoxide on a polysaccharide-based CSP, is explained.
Boosting the Purification Process of Biopharmaceuticals by Means of Continuous Chromatography
June 2nd 2020Single-column (batch) chromatography, involving two or more successive single-column (batch) chromatographic steps, is a standard approach for purifying biopharmaceuticals. Step one, known as the capture step, is used to remove product-related impurities, and step two, the polishing step, is used to remove product-related impurities. Here we present and illustrate the advantages of continuous chromatography for these separations: capture simulated moving bed (captureSMB) for the capture step and multicolumn countercurrent solvent gradient purification (MCSGP) for polishing.