
Understanding the chemistry of embedded polar group and perfluorophenyl phases leads to better decisions during method developement

Understanding the chemistry of embedded polar group and perfluorophenyl phases leads to better decisions during method developement

Many problems encountered executing HPLC methods are a result of decisions made during early method development. This article discusses one critical variable in method development, the choice of stationary phase chemistry.

Vitamin D deficiency has become a topic of interest in recent publications (1–3). Vitamin D is present in two forms, Vitamin D3 and Vitamin D2. D3 is produced after ultraviolet light-stimulated conversion of 7-dehydrocholesterol in the skin (3).

Published: June 1st 2011 | Updated:

Published: September 1st 2014 | Updated:

Published: September 1st 2014 | Updated: