Significant recent advances now enable routine usage of HDX-MS for comparing the conformations of biopharmaceutical products.
In this column, we introduce the basics of today’s approaches for doing intact protein dissociation with mass spectrometry (MS), or top-down sequencing (that is, rather than the more conventional peptide-based “bottom-up” sequencing where future improvements might occur, advantages and limitations of using top-down sequencing, possible applications, and why it has become such an important and pursued research area for many.
A commentary on maintaining and growing technical competencies and the effort that is required.
The modern usage and specific applications of HILIC for two other major classes of analytes - glycopeptides and glycoproteins - are discussed.
Answers to common questions about HILIC, and an overview of its application to biopharmaceutical analysis.
How to use these tools to develop an efficient and robust process