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This article demonstrates the suitability of ultrapure water from a Milli-Q® water system equipped with VOC-Pak® point-of-use cartridge for sensitive VOC analysis. This water is free of 58 VOCs evaluated, making it optimal for Purge and Trap GC-MS analysis of these compounds.

Quantitative measurement of biotherapeutic and clinically important biomarker proteins using LC-MS/MS has seen great development in the last 15 years. Stable Isotope Labelled Internal Standards are a cornerstone of accurate quantitation and there are several options ranging from signature peptides to “winged” longer peptides or full 13C and/or 15N labelled proteins to choose from. In this video Kevin Ray, Kwasi Mawunyega and Anders Fridstrom will discuss pros and cons with different SIL IS approaches as well as similarities and differences between biopharmaceutical bioanalysis and biomarkers for disease research & diagnosis.

Experts will be sharing their experience in providing tips for protein biologics sample preparation. Special focus is on reduction and deglycosylation for characterizing antibody-drug conjugates, as well as enzyme and chemical treatment options to ensure desired results when deglycosylating glycoprotein samples.

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