Experimental data are presented to help understand HPLC column retention in a way that should make column selection and method development proceed faster when initial results with C18 columns are disappointing.
In the second of a two-part series, Marlin K.L. Bicking continues to explain his work concerning integration errors in peaks with approximately equal sizes (small peak ratios).
In part I of a two-part series, the author introduces several methods to reduce error in your chromatographic analyses.