Pavel Jandera | Authors

Pavel Jandera spoke to Frantisek Foret about building his own liquid chromatograph, the birth of “modern” high performance liquid chromatography (HPLC), the importance of exploring (and understanding) earlier research papers, current trends in contemporary chromatography, and his inspiring advice for aspiring chromatographers.

Recent developments have made organic polymer monoliths much more suitable for the separation of small molecules.

Developments in the preparation of organic polymer monoliths suitable for the separation of small molecules in the isocratic mode are described and the main factors affecting column efficiency are identified.

This article describes the factors that affect the selection of columns for two-dimensional (2D) LCxLC separations.

Proteins can be separated according to their isoelectric points on an ion-exchange column using pH gradients in the first dimension and according to their hydrophobicity on an RP column in the second dimension.

This article describes the factors that affect the selection of columns for two-dimensional (2D) LCÃ-LC separations. The maximum increase in peak capacity compared with single-dimension (1D) separations is obtained by using "orthogonal" systems employing various combinations of separation mechanisms to provide as different separation selectivities as possible for the sample compounds in the first and in the second dimension systems. To obtain best results, matching the chemistry of the stationary phase, column dimensions and mobile phases in the first and in the second dimensions is essential for successful separations, especiall for comprehensive LCÃ-LC.