Reiko Kiyonami | Authors

Reiko Kiyonami is with Proteomics Marketing Thermo Fisher Scientific, San Jose, California.


Large-Scale Targeted Protein Quantification Using Wide Selected-Ion Monitoring Data-Independent Acquisition

This article describes the development of a new data-independent acquisition (DIA) workflow for protein quantification that uses a mass spectrometer that combines three types of mass analyzers to achieve lower limits of detection (LOD), higher sensitivity, more accurate quantitative results, wider dynamic range, and better reproducibility than existing high-resolution accurate-mass (HRAM) tandem mass spectrometry (MS-MS) DIA workflows.

Targeted Protein Quantification Using High-Throughput Capillary LC–MS

The detection limit, analytical precision, dynamic range, and robustness of a method for the targeted quantification of peptides using a capillary-flow LC–MS system were evaluated by spiking known amounts of isotopically labeled yeast peptides into a 500-ng yeast digest matrix.

Targeted Quantitative Protein Analysis in Human Serum Using High-Resolution Multiple Selected Reaction Monitoring Assays

A common endpoint for a biomarker discovery experiment is a list of putative marker proteins. The next step is then to perform targeted quantitative measurements of these proteins in an expanded patient population to assess their validity as markers. Analytical accuracy and precision are required for unambiguous quantitative analysis of targeted proteins from very complex mixtures. Wide dynamic range and high sensitivity are critical for detecting low-abundance proteins. Such an assay also is appropriate for "targeted discovery" experiments, where the goal is to quantitate a large number (up to hundreds) of known proteins in a complex sample.